Vitamins in native tree pollens are essential to support fitness of wild-caught Bombus spp. queens, with positive carry-over effects

<jats:p>To implement adequate nutritional landscapes to bumble bees and inform conservation efforts, we need a better understanding of how different pollen sources impact bumble reproductive performances and phenology. We assessed the impact of multiple native pollen on bees reproductive success by feeding 40 wild-caught Bombus impatiens and B. griseocollis queens with either commercially wildflower pollen mix commonly used to breed Bombus queens (control group) or 75% red maple pollen (Acer rubrum) and 25% pollen mix (RM group). In a second experiment, we monitored the reproductive success of 45 microcolonies of workers, harvested from B. impatiens colonies. Microcolonies were fed either: 1) pollen mix (N=15), 2) pollen mix and hawthorn (Crataegus canadensis, N=15), or 3) pollen mix and sumac (Rhus typhina, N=15). In experiment 1, RM significantly increased reproductive outputs for both species. RM-fed founding queens were twice more likely to produce workers, and produced them significantly earlier, though total number of workers did not vary between groups. However, RM-fed founding queens produced significantly more brood cells, males and gynes than control colonies, and produced them significantly earlier. In experiment 2, we found an interaction between initial colony diet and current microcolony diet. Overall, workers harvested from RM-fed colonies produced significantly more brood cells and males than workers harvested from control colonies. However, when fed hawthorn pollen during experiment 2, workers from control colonies performed as well as workers from RM-fed colonies. This suggests carry-over effects from early pollen sources provided to colonies on workers, which may be circumstantially offset by pollen sources with specific nutrient profile. Using detailed nutritional information of each pollen (including data on 32 nutrients), we provide nutritional requirements of pollen to support bumble bee reproduction, especially regarding vitamin B3 and vitamin C contents. This study will help inform land managers and captive breeding programs on the ideal pollen sources for bumble bees to ensure reproductively successful colonies.</jats:p>