Abstract
<jats:p> Viral diagnostics during early phases of infection are often limited by target scarcity and the deployment tempo. We significantly advance both quantitative accuracy and diagnostic throughput of viral agglutination assays with Immuno-Janus Particle (IJP) aggregation behavior that "flicker" stochastically with size-dependent statistics. By scrutinizing microscale blinking patterns of time series fluorescent videos, we decipher Brownian dynamics of individual IJP-Virus conjugates and IJP aggregates via windowed Itô stochastic analysis (termed the Culsans method). High-frequency rotational fluctuation is deconvolved from corrupting drifts caused by gravitational sedimentation and Brownian translational motion. This methodology enables a non-linear mapping of angular positions of detected IJPs and IJP aggregates to extract rotational diffusivity (D <jats:sub>r</jats:sub> ) (and subsequently overall construct size) with superior linearity (R <jats:sup>2</jats:sup> ≥0.85). The aggregation behavior exhibits a maximum when the IJP and viral particle concentrations are equal. The virion-bridged IJP-IJP conjugates significantly shift the detectable hydrodynamic diameter in the Poisson limit of reduced virus concentration with respect to IJPs, pushing the limit of detection (LOD) to 10 <jats:sup>3</jats:sup> - 10 <jats:sup>4</jats:sup> virions per mL in untreated human plasma. This tunable platform offers a rapid, low-volume, and scalable alternative to lab-based RT-PCR, bridging the gap between virion sensitivity and field-readiness. </jats:p>