Abstract
<jats:p>Reducing postharvest fruit loss without compromising fruit quality is a major goal in tomato breeding. Fruit shelf life is a complex trait, influenced by postharvest changes in fruit firmness and weight, as well as by both genetic and environmental factors. A QTL mapping experiment was conducted to identify loci associated with fruit shelf life-related traits using two distinct F₂ tomato populations. Fruit weight, firmness, shelf life (evaluated as both loss of fruit weight and loss of firmness over time), and colour space components were measured, and QTLs were mapped using a commercial low-density SNP genotyping panel and bulk segregant sequencing analysis experiments. We show that fruit firmness at harvest is only weakly predictive of postharvest firmness loss, indicating that shelf life should be treated as a dynamic trait rather than a static firmness phenotype. Across the two populations, 60 QTLs defining 26 genomic regions were identified, including both population-specific loci and shared regions on chromosomes 9 and 12. Several narrow intervals contained candidate genes related to ethylene signaling, cell-wall remodeling, calcium transport, aquaporin-mediated water balance and stress responses. The identified QTLs and candidate genes are directly relevant to breeding programmes seeking to improve postharvest performance without using major ripening mutants that compromise fruit quality.</jats:p>