Abstract
<jats:p>Chemical exchange refers to a system in macroscopic equilibrium, which at a microscopic level undergoes a dynamic process resulting in one nucleus exchanging its environment with another. Its measurement is circumscribed by spin lattice (T1) and spin-spin (T2) relaxation of the system. Singlet states, which are nuclear spin states with lifetimes greater than T1 and T2 , can be applied to increase the range of such processes measured by NMR. Singlet state two-dimensional exchange spectroscopy was earlier used to qualitatively detect exchange in a system showing slow dynamics, but its use for quantitative study of the kinetics and thermodynamics of chemical exchange was not demonstrated. In this research work, a method involving singlet states is applied to extract kinetic and thermodynamic information of chemical exchange. It is applied to a prototypical example, p-chloroformanilide. As a fallout, the presence of two isomeric forms of p-chloroformanilide in acetone was detected contrary to earlier reports.</jats:p>