Production of Diagnostic Antibodies against exotoxin A of Pseudomonas aeruginosa., using Phage Display Technique

<title>Abstract</title> <p> Patients with compromised immune systems are particularly susceptible to nosocomial infections caused by <italic>Pseudomonas aeruginosa</italic> . Through ADP-ribosylation of eukaryotic elongation factor 2, <italic>Pseudomonas aeruginosa</italic> exotoxin A inhibits protein synthesis in infected cells. Plasmodium infections, even when resistant to antibiotics, can be treated by using antibodies that neutralize the virulent components of the bacteria. <italic>Pseudomonas aeruginosa</italic> produces exotoxin A, which is highly effective in treating <italic>Pseudomonas aeruginosa</italic> infections. For this research. An antibody specific to P. aeruginosa exotoxin A was identified by mining a human scFv phage library. In order to induce protein injection in mice, exotoxin A was produced in Escherichia coli. The scFv repertoires were constructed by combining the coding sequences of the Ig G variable domain. They originated from RNA found in the liver. We prepared a three-phage library using scFv using 1×10 ⁵ plaque forming units. The characteristics of the library demonstrated the production of 31.67-kDa bacterial proteins with good selectivity and affinity scFvs. </p>