Abstract
<title>Abstract</title> <p> <italic>Identification and characterization of the isolated and screened amylase-producing bacteria, either up to the species or genus level, is the primary step before using them for amylase production. The aim of this study is to identify and characterize by morphological, physiological, and some biochemical tests at the genus level because the soil is a rich source of microorganisms that secrete amylase.</italic> <italic>From 5 isolates, 2 prescreened amylase-producing bacterial isolates from sewage water (w3 and w5) and 3 prescreened amylase-producing bacterial isolates from soil receiving kitchen waste (s3, s4, s5) were subjected to morphological, biochemical, and physiological tests. All the data was analyzed and organized by using a Microsoft Excel spreadsheet and presented by table and graph. In the present study, amylase-producing bacterial isolates were varied in morphological character. Almost all amylase-producing bacterial isolates showed different biochemical characteristics. According to Bergey's manual, the isolates genera identified as Bacillus, Yersinia, and Providencia. The majority of bacterial isolates were motile, gram-positive spore producers, and they also showed different biochemical characteristics. The isolates were able to grow in different temperatures, pH, salinity, and starch concentration, but the optimum growth was observed as 37°C, pH5, 1% NaCl concentration, and 7% starch concentration. The growth of amylase producing bacterial isolates and the concentration of NaCl were inversely proportional, but the growth of isolates has a leaner relationship with starch concentration. It recommended that sewage water and soil receiving kitchen waste could be used as a source of amylolytic bacteria for amylase enzyme production.</italic> </p>