Abstract
<title>Abstract</title> <p> Carbapenemase-producing Enterobacterales (CPE) are critical-priority antimicrobial-resistance pathogens, but their genomic visibility across One Health compartments remains uneven. We analysed records from the NCBI Pathogen Detection Isolates Browser, which integrates pathogen genomes, isolate metadata and AMRFinderPlus-derived AMR genotypes. Records carrying NDM, KPC, VIM, IMP or OXA-48-like carbapenemase families were retained for target Enterobacterales organism groups. After source curation, 139,466 CPE records and 149,473 family-level records were analysed. Human/clinical records dominated (118,156; 84.72%), while records from wastewater (2.54%), other environmental sources (1.48%), animals (1.30%), water/environment (0.25%) and food (0.09%) were much less common. Source category and carbapenemase family were associated (chi-square = 6107.37, df = 24, P < 0.001; Cramer's V = 0.101). Animal-associated NDM enrichment was driven mainly by <italic>Escherichia coli/Shigella</italic> and <italic>Klebsiella pneumoniae</italic> , whereas wastewater-associated KPC enrichment was led by <italic>K. pneumoniae</italic> , <italic>Enterobacter cloacae</italic> and <italic>Citrobacter freundii.</italic> These findings show that public genome data can reveal One Health patterns, but that record counts reflect repository composition rather than prevalence. </p>