Abstract
<title>Abstract</title> <p> Objective Hospital-acquired infections (HAIs), particularly in intensive care units of pediatric department, pose a significant global health risk due to their link with multidrug-resistant organisms, increased morbidity, and death. The burden of HAIs is exacerbated in resource-constrained locales such as Agartala, Tripura, due to inadequate healthcare resources and a large influx of patients from rural areas. However, thorough research on the epidemiology of HAIs in such settings is limited. <bold>Methodology</bold> : The study involved patients hospitalized for over two days and presenting new symptoms in the PICU and NICU of AGMC & GBP Hospital. Blood samples were collected using dual-sampling techniques, incubated in BHI broth, and sub-cultured on MacConkey and blood agar plates. Antibiotic susceptibility testing was performed on Mueller-Hinton agar using the Kirby-Bauer method, with control strains <italic>E. coli</italic> ATCC 25922 and <italic>S. aureus</italic> ATCC 29213 ensuring quality. Result Out of 50 samples, 26% were positive for bloodstream infections, with 92.3% being primary BSIs. <italic>Staphylococcus aureus</italic> was the most prevalent pathogen, with 100% sensitivity to linezolid and vancomycin. Longer hospital stays and longer recovery durations suggest the need for timely interventions to improve outcomes. Conclusion The study highlights the high burden of bloodstream infections in pediatric intensive care, primarily caused by <italic>Staphylococcus aureus.</italic> It emphasizes the importance of linezolid and vancomycin in treatment, the correlation between hospital stays and recovery durations, and the need for early diagnosis and intervention. </p>