Abstract
<title>Abstract</title> <p>The technologies for implant fabrication are improving every year. Currently, there is a significant demand for implants that not only exhibit high biocompatibility but also possess antibacterial, antiresorptive, and antitumor properties. In this study, we present a new method for creating implants based on the Ti6Al4V alloy, which surface was modified using plasma electrolytic oxidation followed by the formation of a polydopamine layer with bioactive substances. Vancomycin was used as the antibacterial component of the coating, while bisphosphonate (zoledronic acid) served as the antiresorptive agent. The presence of zoledronic acid and vancomycin in the surface layers was confirmed using X-ray photoelectron spectroscopy, scanning electron microscopy combined with energy-dispersive spectroscopy, and Raman spectroscopy. During MTS analysis, the culture medium extracted from the samples exhibited cytotoxic activity against human osteosarcoma cells (MNNG/HOS). The data obtained suggests the successful formation of a coating based on the titanium alloy and demonstrates the bioactive properties of the surface layers.</p>