Figure 3 from An Integrated Spatial Multi-Omics Workflow for Sequential RNA and Protein Profiling in FFPE Tumor Tissue

<jats:p>&lt;p&gt;Application of sequential spatial multi-omics imaging and standard IMC workflow on serial sections of CRLM. Top, expression of key protein markers keratin 8/18 (&lt;b&gt;A&lt;/b&gt;), α-SMA (&lt;b&gt;B&lt;/b&gt;), CD45 (&lt;b&gt;C&lt;/b&gt;), and vimentin (&lt;b&gt;D&lt;/b&gt;) on serial tissue sections on an ROI from tumor 3. Bottom, classification of segmented cells based on the expression pattern of the selected protein markers, generated using QuPath. &lt;b&gt;E,&lt;/b&gt; Quantitative assessment of the mean expression of the protein markers in the multi-omics and single-modality IMC workflow, depicting that sufficient protein signal intensity is maintained in the multi-omics workflow to distinguish between marker-positive and marker-negative cell populations.&lt;/p&gt;</jats:p>