Figure 6 from Schlafen 11 Is Overexpressed in Multiple Myeloma and Undergoes Nucleolar Translocation in Response to Bortezomib

<jats:p>&lt;p&gt;SLFN11-dependent suppression of rRNA synthesis by BTZ. &lt;b&gt;A,&lt;/b&gt; Representative immunofluorescence images showing total SLFN11 (green, no preextraction) and DAPI (blue) in parental and SLFN11 KO KMS-34 cells. Scale bars, 10 μm. &lt;b&gt;B,&lt;/b&gt; SLFN11 immunoblots for whole-cell lysates from WT and SLFN11 KO clones of KMS-34 cells. &lt;b&gt;C,&lt;/b&gt; Representative immunofluorescence images showing EU incorporation (green) and DAPI (blue). Top, KMS-34 cells. Bottom, KMS-27 cells. WT and SLFN11 KO clones were treated with BTZ (0.1 μmol/L) for indicated time points. Scale bars, 5 μm. &lt;b&gt;D,&lt;/b&gt; Quantification of EU signal intensities in individual cells. Mean ± SEM are shown. Top, KMS-34 cells (threshold = 80). Bottom, KMS-27 cells (threshold = 70). Percentages of cells below threshold are indicated. ****, &lt;i&gt;P&lt;/i&gt; &lt; 0.0001 (one-way ANOVA). a.u., arbitrary units.&lt;/p&gt;</jats:p>