Figure 5 from Microglia Display Heterogeneous Initial Responses to Disseminated Tumor Cells

<jats:p>&lt;p&gt;Dominance of protumor microglia at brain metastasis formation sites in a mouse model. &lt;b&gt;A,&lt;/b&gt; Schematic of microglial depletion using PLX in the DTC model. ICA, internal carotid artery. &lt;b&gt;B,&lt;/b&gt; Representative &lt;i&gt;in vivo&lt;/i&gt; images of Cx3cr1-EGFP and CMT167mCh (mCherry) cells after microglial ablation. The tumor volume on day 12 was smaller in the PLX-treated models. &lt;b&gt;C,&lt;/b&gt; Total tumor size in individual PLX-treated mice. &lt;b&gt;D,&lt;/b&gt; Size of each individual DTC/micrometastasis on days 2 and 12. &lt;b&gt;E,&lt;/b&gt; Schematic representation of &lt;i&gt;Cx3cr1&lt;/i&gt;&lt;sup&gt;&lt;i&gt;GFP/+&lt;/i&gt;&lt;/sup&gt;&lt;i&gt;:Siglech&lt;/i&gt;&lt;sup&gt;&lt;i&gt;DTR/wt&lt;/i&gt;&lt;/sup&gt; models of microglia (MG)-specific depletion. &lt;b&gt;F,&lt;/b&gt; Representative &lt;i&gt;in vivo&lt;/i&gt; images of microglia-depleted (&lt;i&gt;Cx3cr1&lt;/i&gt;&lt;sup&gt;&lt;i&gt;GFP/+&lt;/i&gt;&lt;/sup&gt;&lt;i&gt;:Siglech&lt;/i&gt;&lt;sup&gt;&lt;i&gt;DTR/wt&lt;/i&gt;&lt;/sup&gt;) and &lt;i&gt;Cx3cr1&lt;/i&gt;&lt;sup&gt;&lt;i&gt;GFP/+&lt;/i&gt;&lt;/sup&gt; mice. &lt;b&gt;G,&lt;/b&gt; Trend in total tumor size under microglial ablation in each mouse. &lt;b&gt;H,&lt;/b&gt; Size of each individual tumor on days 2 and 12. &lt;b&gt;I,&lt;/b&gt; Schematic representation of the fate of tumor cells and their microglial roles. Error bars, SD. *, &lt;i&gt;P&lt;/i&gt; &lt; 0.05; **, &lt;i&gt;P&lt;/i&gt; &lt; 0.01; ****, &lt;i&gt;P&lt;/i&gt; &lt; 0.0001; ns, nonsignificant.&lt;/p&gt;</jats:p>