Abstract
<jats:p>Pesticides are chemicals that are extensively utilized in the agricultural sector for the purpose of controlling and/or kill population of insects, pests, weeds, rodents, fungi, and diseases that have the potential to compromise crop yield and quality. They include several chemical classes, each with distinct mechanisms of action and toxicological profiles. Although these compounds have contributed to pest control in agriculture, they are also associated with harmful and detrimental effects on health, environmental pollution, and loss of biodiversity. Since the introduction of synthetic pesticides in the 1940s, analytical methodologies have evolved from techniques such as gas chromatography and thin‐layer chromatography to current methods based on several LC techniques, mainly coupled with high‐resolution mass spectrometry detectors. Target, suspect, and nontarget analysis have been developed. In addition, innovations in off‐line and on‐line pretreatment methods and the development of multi‐residue analysis have optimized the simultaneous detection of multiple compounds. In recent years, the incorporation of miniaturized LC and portable LC has opened new perspectives for faster analysis, reflecting the growing scientific interest in this field. The development of green and sustainable methodologies is a goal.</jats:p>